막성 및 연골성 탈회골기질에 의한 골유도와 재생에 관한 연구
Bone Induction nd Regenera6ion by Membranous and Endochondral Demineralized Bone Matrix
소속 상세정보
김선헌
김호철/김민석/황인남/오원만/이은주
KMID : 0355819950190010125
Abstract
Since bone matrix is known to contain osteoinductive substance of form bone by endochondral ossification, there are much clinical interests concerning it. But there are still controversies about clinical effects, the methods of osteoinduction and
preparations of bone matrix. About 700㎛demineralized endochondral and intramembranous bone matrix particles, which have different origin in development were made from long bone and calvaria of rabbit respectively. They were allografted into the
artificial defects of medial surface of tibia. The mode of bone formation and regeneration are stuided by microscopic and fluorescent observation.
1. A lot of fibrin network formed among the demineralized bone particles after 3 days of graft.
2. Lost of spindle shaped cells appeared around the particles and begin to bone formation 7 days after graft. bone formation was mainly taking place from the host bone in control group.
3. Active bone induction by endochondral and intramembranous bone formation from both kinds of the matrix particles, which were being resorted by multinucleated cells, was noted.
4. More rapid bone regeneration and maturity were seen in demineralized bone grafted groups than in control in grinding preparations and fluorescent microscopy.
5. There is no considerable difference of osteoinductivity between the membranous and endochondral bone matrices.
These results suggest that endochondral bone formation is not critical in osteoinduction by demineralized bone maritices and both types of matrices and both types of matrices can be applicated clinically in bone graft.
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